BIOBANK CO., LTD. (located in Okayama City, Okayama Prefecture) has conducted a collaborative research with associate professor Tomohiro Itoh and his colleagues from the Graduate School of Bioresources at Mie University (located in Tsu City, Mie Prefecture) and discovered that the fermented vegetable extract (OM-X) contributes to suppress fatigue and enhance muscle endurance.
The results have been published in the first issue of the Natural Product Communications in 2017.
About 60% of the working population in Japan is aware of the feeling of fatigue and a half of them are distressed by symptoms of chronic fatigue (lasted for over 6 months) (1). At present, a number of studies pertaining to recovery from fatigue have been progressing and some studies have reported that natural ingredients such as imidazole dipeptide in chicken breast and astaxanthin in fish are effective (2, 3) for relieving fatigue.
BIOBANK CO., LTD. develops and produces an extract (OM-X) that was made with fermented vegetables, fruits, seaweeds, mushrooms and other ingredients. Many OM-X users have given feedbacks saying that “they feel less tired after having OM-X”. Therefore, we started think about the potential of OM-X in relieving fatigue.
Based on these backgrounds, BIOBANK CO., LTD. conducted a collaborative research with associate professor Tomohiro Itoh in order to confirm the potential of OM-X in relieving fatigue and understand its mechanism.
As a result of a forced swimming test among mice, the swimming duration of the mice with OM-X is two times longer than the mice without OM-X. We analyzed the liver of mice and found an increased gene expression level of enzymes which are used for the metabolism of fatigue-related substances named ammonia. Therefore, we considered that the metabolism of fatigue-related substances was improved because of the intake of OM-X which in turn extended the swimming duration of the mice. The study results suggested that the fatigue-relieving effect was exerted and muscle endurance was improved by administering OM-X to the mice.
We performed a forced swimming test with mice expecting to see the fatigue-relieving effect of OM-X.
We separated mice into 2 groups as one group was given OM-X and the other group was not dosed with OM-X. The group with OM-X was given 85 mg/kg of their body weight for 4 weeks continually and was forced to swim every week for 4 weeks of the test period. The group without OM-X was also forced to swim following the same parameters as the group with OM-X. As a result, the swimming duration of the mice with OM-X became approximately two times longer than the mice without OM-X. The fatigue-relieving effect and the enhancement of muscle endurance have been confirmed through this test (Chart1).
After the forced swimming test, we analyzed the serum components and confirmed the decrease in concentration level of lipid components (cholesterol, neutral lipid and phosphatide) in serum and the liver of the mice with OM-X. This means that the lipid components have been consumed during exercise. In addition, the quantity of lactic acid in serum decreased. This suggested that lactic acid is reused as a source of energy during heavy exercise.
Furthermore, after analyzing the gene expression of enzymes Arg1 and Cps1 which detoxify ammonia: a cause of fatigue, we confirmed that the gene expression level of the group with OM-X was higher than the group without OM-X (Chart2).
According to these research results, an accumulation of fatigue was suppressed among the mice which were dosed with OM-X and their duration of exercising was elongated. These results encourage us to expect OM-X to be a helpful food/material for symptomatic relief of fatigue and an enhancement of muscle endurance.
【Introduction of instructor Tomohiro Itoh Ph.D.】
Name: Tomohiro Itoh
Affiliation: Mie University, Graduate School of Bioresources, Marine Biotechnology Course
1998 Graduated from National Fisheries University
1998 ~ IMURAYA GROUP CO., LTD. Central Institute in position of researcher
2006 ~ Gifu Prefectural Global Institute of Biology Healthy Useful Substance Research Department in position of researcher
2007 ~ Gifu Prefectural Global Institute of Biology Healthy Useful Substance Research Department in position of research specialist
2011 ~ Kinki University, Faculty of Agriculture Fisheries Departments in position of instructor and associate professor
~ Mie University, Graduate School of Bioresources in position of associate
professor until present
Last updated 2018/02/19