Evaluation of therapeutic properties of fermented vegetables extract (OM-X) in the model of colitis induced by Citrobacter rodentium in mice
Case：Enteritis / Immunity / Infection
The fermented extract OM-X contributed to suppress the inflammatory reactions in intestines and recovered intestinal epithelial cells of the infected mice with the pathogen
J Functional Foods. 10, 117-127 (2014)
The fermented extract
OM-X contributed to suppress the inflammatory reactions in intestines and
recovered intestinal epithelial cells of the infected mice with the pathogen
The results of a collaborative research with University of
Lille Nord de France have been published in the Journal of Functional Foods.
We examined the anti-inflammatory effect of the OM-X extract
by using mouse models with bowel inflammation induced by the infection with Citrobacter
rodentium which is a mouse pathogen.
We prepared mouse models with bowel inflammation induced by Citrobacter
rodentium in both groups with and
without administration of the OM-X extract in order to evaluate the
anti-inflammatory effect of the OM-X extract. After completion of the dosing period, we observed the subject
groups with respect to enteritis scores and gene expressions of immune cells
which are involved in inflammation and anti-inflammatory activities.
The group that was given the OM-X extract and infected with
the pathogen showed a tendency to reduce the inflammation score.
Once the subjects are infected with pathogens, they trigger
overactive immune responses which in turn invoke inflammation. The group that
was given the OM-X extract before the inoculation of the pathogen showed a
significant increment in FoxP3 expression; the index of Treg cells which play
an important role in anti-inflammatory activities. Moreover, the expression of
IL-10 which is an anti-inflammatory cytokine significantly increased.
We examined the contribution of the OM-X extract for the
recovery of epithelial cells in the intestine of mouse models with a leaky gut
symptom induced by the infection with mouse pathogen.
We destroyed the epithelial cells in the mice intestines and
administered orally Citrobacter rodentium in order to prepare the mouse
models with a leaky gut symptom. Then,
we confirmed the conditions of the epithelial cells through the
examinations on the gene expression that is involved in the tight junctions and
the cellular proliferative potential by pigmenting the epithelial cells.
The group that was given the OM-X extract showed a
significant increment of the gene expression of ZO-1 and ZO-2 proteins
associated with tight junctions where they join two cells
together in the intestines.
time of infection with the pathogen, the control group showed little
pigmentation on the cells indicating the cellular proliferative potential, but
the group that was given the OM-X extract showed a lot of pigmentation on the
cells. These results suggested that the destructed epithelial cells in the intestines
recovered over the active cellular proliferative.
Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces
Case：Identification of LAB
Determination of complete sequence of plasmids contained in Bifidobacterium kashiwanohense JCM 1543
Anim Sci J. 85, 158-163 (2014)
The results of a collaborative research with School of
Veterinary Medicine, Azabu University have been published in the Animal Science
We analyzed 2 plasmids contained in bifidobacterium
which were isolated from a healthy infant.
We cultured Bifidobacterium kashiwanohense JCM
15439 isolated from a healthy infant and purified plasmids
pBBKW-1 and pBBKW-2. Subsequently, we determined and analyzed a complete
sequence of these plasmids. We also performed an analysis with Southern-hybridization.
The replicated plasmid pBBKW-1 was a rolling
circle-type and replicated pBBKW-2 was a θ-type which has a better stability than a rolling
Anim Sci J. 85, 158-163 (2014)
The results of a joint research with School of Veterinary Medicine, Azabu University have been published in the Animal Science Journal. The research results show discovery of a new strain of bifidobacteria from healthy infants and information of plasmids this bifidobacteria holds.
Safety and tolerability of Dr Ohhira’s OM-X Capsules in healthy volunteers
Safety of Dr. Ohhira’s OM-X capsules in human
Integrative Medicine. 12, 38-42 (2013)
The results of a collaborative testing over safety on human
with Harvard Medical School (USA) using Dr. Ohhira’s OM-X capsules in healthy
volunteers have been published in IMCJ.
We performed a safety study in humans
using Dr. Ohhira’s OM-X capsules
The study subjects were 46 healthy adults. The subjects took
one OM-X capsule twice a day for 30 days. We determined if the person has a
serious condition at the second day of the test and after the test was
completed. The test was done with a double blind method.
adverse effects were observed in the group who took the OM-X capsules. Safety of
the OM-X capsules on human was confirmed.